3130 Highland, Cincinnati, OH Hoxworth Blood Center, Research Division, College of Medicine, University of Cincinnati

Publications

TTS
Develop Tandem Tension Sensor to Gauge Integrin-transmitted Molecular Forces

G. Niraula*, A. Pyne* and X. Wang

 * Equal contribution

ACS Sensors

2024

Accepted

FA force
Develop a ratiometric tension sensor exclusively responding to integrin tension magnitude in live cells

A.Sarkar*, G. Niraula*, D. N. LeVine, Y. Zhao, Y. Tu, K. Mollaeian, J. Ren, L. Que and X. Wang

 * Equal contribution

ACS Sensors

2023

Link

FA force
Vinculin transmits high-level integrin tensions that are dispensable for focal adhesion formation

J. Austin, Y. Tu, K. Pal and X. Wang

Biophysical Journal

2023

Link

ITS
Imaging Cell Adhesive Force at the Single Molecule Level

Y. Tu and X. Wang

Methods Molecular Biology, Vol. 2600

2022

Book chapter

Filopodia adhesive force
Filopodial adhesive force in discrete nodes revealed by integrin molecular tension imaging

Y. Tu, K. Pal, J. Austin and X. Wang

Current Biology

2022

Link

Podosomal integrin tension
Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Ligand Tensions or Interactions

K. Pal, Y. Tu and X. Wang

ACS Nano

2022

Link

Cell migration track
Tracking cell migration by cellular force footprint recorded with a mechano-optical biosensor

Y. Tu and X. Wang

Biosensors and Bioelectronics

2021

Link

Lab on a chip
Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear stress and ROCK inhibitor on platelets

S. Mao*, A. Sarkar*, Y. Wang, C. Song, D. N. LeVine, X. Wang** and L. Que**,  ** Equal contribution

Lab on a chip

 

2021

Link

DNase in podosomes
Ubiquitous Membrane-bound DNase Activity in Podosomes and Invadopodia

K. Pal, Y. Zhao, Y. Wang and X. Wang

Journal of Cell Biology

2021

Link

Tension sensor review
Recent Advances in Cell Adhesive Force Microscopy

Y. Tu and X. Wang

Sensors

2020

Link

Ligand-depleted migration
Cell migration driven by self-generated integrin ligand gradient on ligand-labile surfaces

A. Sarkar, D. N. LeVine, N. Kuzmina, Y. Zhao and X, Wang

Current Biology

2020

Link

Super-resolution cellular force nanoscopy
Cellular force nanoscopy with 50 nm resolution based on integrin molecular tension imaging and localization

Y. Zhao, K. Pal, Y. Tu and X. Wang

Journal of the American Chemical Society

2020

Link

DNase-resistant tension sensor
Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance

Y. Zhao, A. Sarkar and X. Wang

Biosensors and Bioelectronics

2020

Link

JOVE
Imaging integrin tension and cellular force at submicron resolution with integrative tension sensor

Y. Zhao, N. Wetter and X. Wang

Journal of Visualized Experiments

2019

Link

DNase on cancer cell membrane
Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane

Y. Wang, Y. Zhao, A. Sarkar, X. Wang

Journal of Biophotonics

2019

Link

Integrin tension during keratocyte migration
Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration

Y. Zhao, Y. Wang, A. Sarkar and X. Wang

iScience

2018

Link

Molecular coating for cellular force imaging
Force-activatable coating enables high-resolution cellular force imaging directly on regular cell culture surfaces

A. Sarkar, Y. Zhao, Y. Wang and X. Wang

Physical Biology

2018

Link

Single cell SiMPull
Towards single-cell single-molecule pull-down

X. Wang, S. Park, L. Zeng, A. Jain and T. Ha

Biophysical Journal

2018

Link

Platelet force map
Force-activatable biosensor enabling single platelet force mapping directly by fluorescence imaging

Y. Wang, D. N. LeVine, M. Gannon, Y. Zhao, A. Sarkar, B. Hoch and X. Wang

Biosensors and Bioelectronics

2018

Link

Multiplexed TGT
Integrins outside focal adhesions transmit tensions during stable cell adhesion

Y. Wang and X. Wang

Scientific Reports

2016

Link

 

Prior to ISU

Z. Rahil, S. Pedron, X. Wang, T. Ha, B. Harley and D. Leckband, Nanoscale mechanics guides cellular decision making. Integrative Biology, 8 (9):929-35 (2016)

M. K. Lee, J. Park, X. Wang, M. Roein-Peikar, E. Ko, E. Qin, J. Lee, T. Ha and H. Kong, “Rupture force of cell adhesion ligand tethers modulates biological activities of a cell-laden hydrogel”, Chemical Communications, 52, 4757, (2016)

F. Chowdhury, I. T. S. Li, T. T. M. Ngo, B. J. Leslie, B. C. Kim, J. E. Sokoloski, E. Weiland, X. Wang, Y. R. Chemla, T. M. Lohman, T. Ha, "Defining Single Molecular Forces Required for Notch Activation Using Nano Yoyo"  Nano Letters, 16(6), 3892-2897 (2016)

X. Wang, Z. Rahil, I. T. S. Li, F. Chowdury, D. Leckband, Y. Chemla and T. Ha, “Constructing modular and universal single molecule tension sensor using protein G to study mechano-sensitive receptors”, Scientific Reports, 6, 21584 (2016)

M. Roein-Peikar, Q. Xu, X. Wang, and T. Ha, “Ultrasensitivity of cell adhesion to the presence of mechanically strong ligands”, Physical Review X, 6, 011001, (2016)

X. Wang*, J. Sun, Q. Xu, F. Chowdury, M. Rokhai, Y. Wang and T. Ha, “Integrin Molecular Tension within Motile Focal Adhesions”, Biophysical Journal, 109, 2259-2267 (2015) (* corresponding author)

F. Chowdhury, I. T. S. Li, B. J. Leslie, S. Doganay, R. Singh, X. Wang, J. Seong, S. H. Lee, S. Park, N. Wang and T. Ha , "Single molecular force across single integrins dictates cell spreading ", Integrative Biology, 7, 1265-1271 (2015)

X. Wang and T. Ha, "Defining Single Molecular Forces Required to Activate Integrin and Notch Signaling", Science, 340, 991-994 (2013).
Selected in Methods to Watch, "Tiny tools to measure force", Nature Methods, 11, 29 (2014).
Selected in Research Highlights, "The Pull of a Cell", Nature Methods, 10, 696-687 (2013).

X. Wang, M. Zhao, and D. D. Nolte, "Molecular layer detection on a diffractive optical balance", Optics Letters, 37, 4098-4100 (2012).

X. Wang, M. Zhao, D. D. Nolte, and T. L. Ratliff, "Prostate specific antigen detection in patient sera by fluorescence-free BioCD protein array", Biosensors & Bioelectronics, 26, 1871-1875 (2011).

X. Wang, M. Zhao, and D. D. Nolte, "Refractive index and dielectric constant transition of ultra-thin gold from cluster to Film", Optics Express, 18, 24859-24867 (2010).

M. Zhao, X. Wang, and D. D. Nolte, "Mass-transport limitations in spot-based microarrays", Biomedical Optics Express, 1, 983-997 (2010).

X. Wang, M. Zhao, and D. D. Nolte, "Ambient Molecular Water Accumulation on Silica Surfaces Detected by a Reflectance Interference Optical Balance", Applied Physics Letters, 97, (2010).

D. D. Nolte, M. Zhao and X. Wang, The BioCD: High-Speed Interferometric Optical Biosensor, Integrated Analytical Systems, Springer-Verlag New York, Book Chapter, (2009)

X. Wang, M. Zhao, and D. D. Nolte, "Optical contrast and clarity of graphene on an arbitrary substrate", Applied Physics Letters, 95, 081102 (2009).

Selected in Virtual Journal of Nanoscale Science & Technology.

X. Wang, M. Zhao, and D. D. Nolte, "Prostate-specific antigen immunoassays on the BioCD," Analytical and Bioanalytical Chemistry, 393, 1151-1156 (2009).

X. Wang, Y. P. Chen, and D. D. Nolte, "Strong anomalous optical dispersion of graphene: complex refractive index measured by Picometrology," Optics Express, 16, 22105-22112 (2008).

X. Wang, M. Zhao, and D. D. Nolte, "Land-contrast self-referencing interferometric protein microarray," Applied Physics Letters, 93, (2008).

Selected in Research Highlights, "Protein Interference", Nature Physics, 5, 9 (2009)

X. Wang, M. Zhao, and D. D. Nolte, "Area-scaling of interferometric and fluorescent detection of protein on antibody microarrays," Biosensors & Bioelectronics, 24, 981-987 (2008).

X. Wang, M. Zhao, and D. D. Nolte, "Combined fluorescent and interferometric detection of protein on a BioCD,"Applied Optics, 47, 2779-2789 (2008).

M. Zhao, X. Wang, and D. D. Nolte, "Molecular interferometric imaging," Optics Express, 16, 7102-7118 (2008).

X. Wang, M. Zhao, and D. D. Nolte, "Common-path interferometric detection of protein monolayer on the BioCD,"Applied Optics, 46, 7836-7849 (2007).

X. Wang, Y. Wang, Y. Jiang, and H. Ma, "Two-photon fluorescence anisotropy imaging," Progress in Biochemistry and Biophysics, 32, 161-167 (2005).

Y. Jiang, Y. Wang, Y. Hao, X. Wang, Q. Y. Qiu, Q. F. Wang and H. Ma, "Effect of berberine on the proliferation of human pulmonary carcinoma cells," Chinese Journal of Pathophysiology 21, 2170-2173 (2005).


CONFERENCE PROCEEDINGS

1. X. Wang, M. Zhao, and D. D. Nolte, "A novel concept for protein microarray: land-contrast BioCD," Proceedings of SPIE 7170, 717003 (2009)
2. M. Zhao, X. Wang, and D. Nolte, "Probing mass-transport and binding inhomogeneity in macromolecular interactions by molecular interferometric imaging," Proceedings of SPIE 7188, 71880O (2009)
3. X. Wang, and D. D. Nolte, "Picometrology of Ultra-thin Gold Film by Spinning-Disc Interferometry," Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9, 454-455 (2008)
4. M. Zhao, X. Wang, and D. Nolte, "Molecular Interferometric Imaging Biosensor to Study Molecular Interactions," 2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, Vols 1-9, 1899-1900 (2008)
5. X. Wang, M. Zhao, and D. D. Nolte, "Multiplexed BioCD for prostate specific antigen detection," Proceedings of SPIE 6848, 68480S (2008)
6. M. Zhao, X. Wang, and D. Nolte, "The in-line-quadrature BioCD," Proceedings of SPIE 6447, 64470B (2007)
7. X. Wang, M. Zhao, and D. D. Nolte, "Four-channel optical detection on protein-patterned BioCD," Proceedings of SPIE 6447, 64470L (2007)


 PATENTS (as the primary inventor)

   System with extended range of molecular sensing through integrated multi-modal data acquisition. Issued Patent Number: US 7659968 (Licensed to and commercialized by Perfinity Biosciences, Inc.)

•   Method and apparatus for conjugate quadrature interferometric detection of an immunoassay. Issued Patent Number: US 7787126 (Licensed to and commercialized by Perfinity Biosciences, Inc.)

•   Balanced-quadrature interferometric protein microarray.  WIPO Patent Publication number: WO/2010/039147

Anwesha Sarkar1*, Gopal Niraula1*, Dana LeVine2, Yuanchang Zhao1, Ying Tu1, Keyvan Mollaeian3, Juan Ren3, Long Que4, and Xuefeng Wang1,5