Publications

Lander N. mSphere of Influence: Compartmentalized cAMP signals in American trypanosomes. mSphere. 2024 Feb 28;9(2):e0063523. doi: 10.1128/msphere.00635-23

Chiurillo MA, Carlson J, Bertolini MS, Raja A, Lander N. Gene editing of putative cAMP and Ca²⁺-regulated proteins using an efficient cloning-free CRISPR/Cas9 system in Trypanosoma cruzi. J Eukaryot Microbiol. 2023 Nov-Dec;70(6):e12999. doi: 10.1111/jeu.12999.

Chiurillo MA, Carlson J, Bertolini MS, Raja A, Lander N. Dual localization of receptor-type adenylate cyclases and cAMP response protein 3 unveils the presence of two putative signaling microdomains in Trypanosoma cruzi. mBio. 2023 Jul 21;e0106423. doi: 10.1128/mbio.01064-23.

Teixeira TL, Chiurillo MA, Lander N, Rodrigues CC, Onofre TS, Ferreira ÉR, Yonamine CM, Santos JG, Mortara RA, da Silva CV, da Silveira JF. Ablation of the P21 gene of Trypanosoma cruzi provides evidence of P21 as a mediator in the control of epimastigote and intracellular amastigote replication. Front Cell Infect Microbiol. 2022 18;12:799668. doi: 10.3389/fcimb.2022.799668

Chiurillo MA, Jensen BC, Docampo R. Drug Target Validation of the Protein Kinase AEK1, Essential for Proliferation, Host Cell Invasion, and Intracellular Replication of the Human Pathogen Trypanosoma cruzi. Microbiol Spectr. 2021 31;9(2):e0073821. doi: 10.1128/Spectrum.00738-21

Chiurillo MA, Lander N. The long and winding road of reverse genetics in Trypanosoma cruzi. Microb Cell. 2021 8(9):203-207. doi: 10.15698/mic2021.09.758

Docampo R, Vercesi AE, Huang G, Lander N, Chiurillo MA, Bertolini M. Mitochondrial Ca²⁺ homeostasis in trypanosomes. Int Rev Cell Mol Biol. 2021 362:261-289. doi: 10.1016/bs.ircmb.2021.01.002

Dave N, Cetiner U, Arroyo D, Fonbuena J, Tiwari M, Barrera P, Lander N, Anishkin A, Sukharev S, Jimenez V. A novel mechanosensitive channel controls osmoregulation, differentiation and infectivity in Trypanosoma cruzi. Elife. 2021 Jul 2;10:e67449. doi: 10.7554/eLife.67449

Dos Santos GRR, Rezende Leite AC, Lander N, Chiurillo MA, Vercesi AE, Docampo R. Trypanosoma cruzi Letm1 is involved in mitochondrial Ca²⁺ transport, and is essential for replication, differentiation, and host cell invasion. FASEB J. 2021 Jul;35(7):e21685. doi: 10.1096/fj.202100120RR

Negreiros RS, Lander N, Chiurillo MA, Vercesi AE, Docampo R. Mitochondrial Pyruvate Carrier Subunits Are Essential for Pyruvate-Driven Respiration, Infectivity, and Intracellular Replication of Trypanosoma cruzi. mBio. 2021 Apr 6;12(2):e00540-21. doi: 10.1128/mBio.00540-21

Lander N, Chiurillo MA, Docampo R. Signaling pathways involved in environmental sensing in Trypanosoma cruzi. Mol Microbiol. 2021 May;115(5):819-828. doi: 10.1111/mmi.14621.

Chiurillo MA, Lander N, Vercesi AE, Docampo R. IP₃ receptor-mediated Ca²⁺ release from acidocalcisomes regulates mitochondrial bioenergetics and prevents autophagy in Trypanosoma cruzi. Cell Calcium. 2020 Dec;92:102284. doi: 10.1016/j.ceca.2020.102284.

Taylor MC, Lander N, Yoshida N. Unravelling T. cruzi Biology. Front Cell Infect Microbiol. 2020;10:382. doi: 10.3389/fcimb.2020.00382. eCollection 2020.

Ning Y, Frankfater C, Hsu FF, Soares RP, Cardoso CA, Nogueira PM, Lander NM, Docampo R, Zhang K. Lathosterol Oxidase (Sterol C-5 Desaturase) Deletion Confers Resistance to Amphotericin B and Sensitivity to Acidic Stress in Leishmania major. mSphere. 2020;5(4):e00380-20. doi: 10.1128/mSphere.00380-20.

Faktorová D, Nisbet RER, Fernández Robledo JA, Casacuberta E, Sudek L, Allen AE, Ares M Jr, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget FY, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano JC, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Novák Vanclová AMG, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Jason Smith G, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, von Dassow P, von der Haar T, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, Lukeš J. Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nat Methods. 2020;17(5):481-494. doi: 10.1038/s41592-020-0796-x.

Lander N, Chiurillo MA, Docampo R. CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi. Methods Mol Biol. 2020;2116:177-197. doi: 10.1007/978-1-0716-0294-2_13.

Lander N, Cruz-Bustos T, Docampo R. A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression in Trypanosoma cruzi. Front Cell Infect Microbiol. 2020;10:68. doi: 10.3389/fcimb.2020.00068 eCollection 2020.

Lander N, Chiurillo MA. State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids. J Eukaryot Microbiol. 2019;66(6):981-991. doi: 10.1111/jeu.12747.

Lerchner J, Sartori MR, Volpe POL, Lander N, Mertens F, Vercesi AE. Direct determination of anaerobe contributions to the energy metabolism of Trypanosoma cruzi by chip calorimetry. Anal Bioanal Chem. 2019;411(17):3763-3768. doi: 10.1007/s00216-019-01882-3.

Chiurillo MA, Lander N, Bertolini MS, Vercesi AE, Docampo R. Functional analysis and importance for host cell infection of the Ca²⁺-conducting subunits of the mitochondrial calcium uniporter of Trypanosoma cruzi. Mol Biol Cell. 2019;30(14):1676-1690. doi: 10.1091/mbc.E19-03-0152.

Bertolini MS, Chiurillo MA, Lander N, Vercesi AE, Docampo R. MICU1 and MICU2 play an essential role in mitochondrial Ca²⁺ uptake, growth, and infectivity of the human pathogen Trypanosoma cruzi. mBio. 2019 May 7;10(3):e00348-19. doi: 10.1128/mBio.00348-19.

Lander N, Chiurillo MA, Docampo R. Genome editing by CRISPR/Cas9 in Trypanosoma cruzi. Methods Mol Biol. 2019;1955:61-76. doi: 10.1007/978-1-4939-9148-8_5.

Lander N, Chiurillo MA, Bertolini MS, Storey M, Vercesi AE, Docampo R. Calcium-sensitive pyruvate dehydrogenase phosphatase is required for energy metabolism, growth, differentiation, and infectivity of Trypanosoma cruzi. J Biol Chem. 2018;293(45):17402-17417. doi: 10.1074/jbc.RA118.004498.

Negreiros RS, Lander N, Huang G, Cordeiro CD, Smith SA, Morrissey JH, Docampo R. Inorganic polyphosphate interacts with nucleolar and glycosomal proteins in trypanosomatids. Mol Microbiol. 2018;110(6):973-994. doi: 10.1111/mmi.14131.

Lander N, Chiurillo MA, Bertolini MS, Docampo R, Vercesi AE. The mitochondrial calcium uniporter complex in trypanosomes. Cell Biol Int. 2018;42(6):656-663. doi: 10.1002/cbin.10928.

Lander N, Chiurillo MA, Vercesi AE, Docampo R. Endogenous C-terminal tagging by CRISPR/Cas9 in Trypanosoma cruzi. Bio Protoc. 2017;7(10):e2299. doi: 10.21769/BioProtoc.2299.

Chiurillo MA, Lander N, Bertolini MS, Storey M, Vercesi AE, Docampo R. Different roles of mitochondrial calcium uniporter complex subunits in growth and infectivity of Trypanosoma cruzi. mBio. 2017;8(3):e00574-17. doi: 10.1128/mBio.00574-17.

Lander N, Chiurillo MA, Storey M, Vercesi AE, Docampo R. CRISPR/Cas9-mediated endogenous C-terminal tagging of Trypanosoma cruzi genes reveals the acidocalcisome localization of the Inositol 1,4,5-Trisphosphate receptor. J Biol Chem. 2016;291(49):25505-25515. doi: 10.1074/jbc.M116.749655.

Lander N, Chiurillo MA, Docampo R. Genome editing by CRISPR/Cas9: A game change in the genetic amnipulation of protists. J Eukaryot Microbiol. 2016;63(5):679-90. doi: 10.1111/jeu.12338.

Lander N, Cordeiro C, Huang G, Docampo R. Polyphosphate and acidocalcisomes. Biochem Soc Trans. 2016;44(1):1-6. doi: 10.1042/BST20150193.

Lander N, Li ZH, Niyogi S, Docampo R. CRISPR/Cas9-Induced disruption of Paraflagellar rod protein 1 and 2 genes in Trypanosoma cruzi reveals their role in flagellar attachment. mBio. 2015;6(4):e01012. doi: 10.1128/mBio.01012-15.

Ulrich PN, Lander N, Kurup SP, Reiss L, Brewer J, Soares Medeiros LC, Miranda K, Docampo R. The acidocalcisome vacuolar transporter chaperone 4 catalyzes the synthesis of polyphosphate in insect-stages of Trypanosoma brucei and T. cruzi. J Eukaryot Microbiol. 2014;61(2):155-65. doi: 10.1111/jeu.12093.

Lander N, Ulrich PN, Docampo R. Trypanosoma brucei vacuolar transporter chaperone 4 (TbVtc4) is an acidocalcisome polyphosphate kinase required for in vivo infection. J Biol Chem. 2013;288(47):34205-16. doi: 10.1074/jbc.M113.518993.

Docampo R, Jimenez V, Lander N, Li ZH, Niyogi S. New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists. Int Rev Cell Mol Biol. 2013;305:69-113. doi: 10.1016/B978-0-12-407695-2.00002-0.

Lander N, Bernal C, Diez N, Añez N, Docampo R, Ramírez JL. Localization and developmental regulation of a dispersed gene family 1 protein in Trypanosoma cruzi. Infect Immun. 2010;78(1):231-40. doi: 10.1128/IAI.00780-09.

Rodríguez E, Lander N, Ramirez JL. Molecular and biochemical characterisation of Trypanosoma cruzi phosphofructokinase. Mem Inst Oswaldo Cruz. 2009;104(5):745-8. doi: 10.1590/s0074-02762009000500014.

Peña CP, Lander N, Rodríguez E, Crisante G, Añez N, Ramírez JL, Chiurillo MA. Molecular analysis of surface glycoprotein multigene family TrGP expressed on the plasma membrane of Trypanosoma rangeli epimastigotes forms. Acta Trop. 2009;111(3):255-62. doi: 10.1016/j.actatropica.2009.05.003.